EQUINE BIO-PRF IN SPORTS MEDICINE
When managing sports horses, orthopedic injuries are one of the top problems. The most frequent equine sports-related issues are tendon or ligament injuries, synovitis, and joint arthritis or arthrosis2–5.
Traditional therapies are typically mid- or short-acting steroids, hyaluronic acid, or PSGAG, in combination or as single drugs. These drugs are merely managing the symptoms by reducing inflammation and pain, and do not aim to cure or reverse the injuries caused by the ongoing degenerative disease.
The latest regenerative therapy to be introduced in equine sports medicine is the platelet-rich fibrin (PRF) solution, BIO-PRF, and particularly the concentrated liquid PRF (C-PRF) derivative.
WHY EQUINE BIO-PRF IS THE NATURAL CHOICE
IN EQUINE SPORTS MEDICINE
Autologous Blood Concentrate
Simplicity and ease of use
One huge advantage of Equine BIO-PRF is the easy and quick processing of the final product, making it broadly available to even smaller clinics. Based on the simple setup of a centrifuge and proprietary but fairly standardized vacuum tubes, the treatments are extremely economical.
Safe to use
It is also safe since it´s autologous and without additives. The content of leucocytes furthermore adds to the product’s safety, which is of the greatest importance, since iatrogenic infections after joint injections can be a life-threatening condition in horses.
The complete team of regenerative cells
It is also safe since it´s autologous and without additives. The content of leucocytes furthermore adds to the product’s safety, which is of the greatest importance, since iatrogenic infections after joint injections can be a life-threatening condition in horses. In the Equine BIO-PRF, the entire group of white blood cells is included.
A large part of the growth factors and the majority of the long-term effects of treatment lies specifically in the inclusion and interactions of the white blood cells 11. The white blood cells further contribute to a shortening of the inflammatory phase, jumpstarting the proliferative, and thus the regenerative phase at an earlier time. It is also in this proliferative phase, the formation of new blood vessels (angiogenesis) is contributing to increased and better vascularization of the area. In Equine BIO-PRF, the white blood cells, platelets, and various cytokines are all included in a carefully selected fraction, both retained in and stimulated by the fibrin, thereby allowing for the full regenerative potential to be utilized.
A large part of the growth factors and the majority of the long-term effects of treatment lies specifically in the inclusion and interactions of the white blood cells 11. The white blood cells further contribute to a shortening of the inflammatory phase, jumpstarting the proliferative, and thus the regenerative phase at an earlier time.
It is also in this proliferative phase, the formation of new blood vessels (angiogenesis) is contributing to increased and better vascularization of the area. In Equine BIO-PRF, the white blood cells, platelets, and various cytokines are all included in a carefully selected fraction, both retained in and stimulated by the fibrin, thereby allowing for the full regenerative potential to be utilized.
Promoting M2-like Macrophage Polarizing
Modulate Macrophage phenotype
PRF is able to modulate the polarization of tissue-residing macrophages17–22 and initiate a shift from the destructive M1-like phenotype towards the regenerative M2-like phenotype16,23.
Intrinsic growth factors
The activated platelets, leukocytes, and fibrin matrix in PRF release an elevated amount of growth factors and cytokines, simulating a larger trauma at the site of injection or on lay6–12. In particular, the growth factors PDGF, VEGF, TGF-β, and IGF-1 are important in regeneration and wound healing13,14.
Modulation of inflammation
The release of the growth factors native to the platelets and leucocytes of PRF play a direct role in the modulation of both acute and chronic inflammation, especially by the modulation of tissue-residing activated macrophage polarization 15,16
Stimulate an acute response
The capacity to modulate inflammation, influence macrophage polarization, and stimulate an acute response leading to accelerated healing and regeneration, puts PRF in a unique position as an autologous biological agent for the treatment of chronic inflammation, pain, and damages in joints and tendons, arising from injuries, arthrosis or arthritis.
Interacts on Inflammation
and the Sensory Pathways of Pain
Pain in joints, tendons, ligaments, and muscles, often arise from inflammation affecting the somatosensory nervous system27.
Upon injury, several cell types, including neurons and tissue-residing macrophages, produce soluble pro-inflammatory cytokines, and chemokines that activate surrounding cells8,28–31 and recruit circulating leukocytes, including macrophages, to the site of injury32–34.
Macrophages play a central role
Macrophages have especially significant functions in regulating inflammation and are considered to be common peripheral regulators of neuropathic pain30,34,35. Through the release of inflammatory mediators and interactions with neurotransmitters and receptors, the immune cells form an integrated network with the somatosensory system, coordinating the immune response, tissue healing and modulates the sensory pathways of pain32.
Showing Promising Results
in Cartilage Regeneration,
Meniscus and Tendon Repair
Cartilage and tendons are two of the most avascular and low cell density tissues found in animals, and thus have a very limited potential for repair and regeneration. Left untreated, defects often do not heal at all and stay in a chronic inflammatory state.
The capacity to modulate inflammation, influence macrophage polarization, and stimulate an acute response leading to accelerated healing and regeneration, puts PRF in a unique position as an autologous biological agent for the treatment of inflammation, pain, and damages in joints and tendons, arising from injuries, arthrosis or arthritis.
The introduction of the additive-free liquid PRF, which forms a fibrin clot upon injection, has been a proposed method for the regeneration of cartilage 24,40 and for accelerating the healing of tendons 26.
Drop-by-Drop release – Combination of White Cells, Platelets
and Fibrin ensures a timely release of growth factors
In PRF, the delivery of cytokines and growth factors is done at a rate that is biologically relevant and responding to the needs of the environment17–23. The function of fibrin has in this sense been compared to an irrigation system, where you do not want to drown the plants with large amounts of water in a single shot, but aim at delivering a biologically suitable amount of water and nutrients in a slow and adapted manner.
PDGF, VEGF, TGF-β EGF, fFGF, IGF-1, TGF-α, PAF, thrombospondin, platelet thromboplastin, coagulation factors, serotonin, histamine, hydrolytic enzymes and endostatin
Platelet-Derived Growth Factors
PDGFs (Platelet-Derived Growth Factors) are a family of essential and potent growth factors and chemotactic agents with an important role in wound healing36,37.
Recombinant PDGF is furthermore used in medicine to help heal chronic ulcers and in orthopedic surgery and periodontics as an alternative to bone autograft to stimulate bone regeneration and repair.
Vascular Endothelial Growth Factor
PDGF and VEGF (Vascular Endothelial Growth Factor) work together to establish and increase the blood supply of worn down and ischemic tissue, by promoting the formation of new blood vessels (angiogenesis) .
Transforming Growth Factor Beta
TGF-β (Transforming Growth Factor Beta) is a multi-functional factor important for, amongst other things, the regulation of the inflammatory response. It controls many things, from cell division, differentiation, and controlled cell death of many different cell types along with IGF-1  , to the stimulation of fibroblast production of hyaluronic acid (HA)  and collagen .
Next Generation PRF Solution
Equine Bio-PRF offers you the next level in blood-derived regenerative medicine. We are pushing the limits of Platelet Rich Fibrin based on in-depth research from our global research team. We are offering you solutions with significantly increased cell content and cell distribution as well as a wast extension of PRF resorption time all based on state-of-the-art research and technology.
Concentrated PRF (C-PRF) the new benchmark
As our research team gained insight on how cells distribute across a fibrin clot, a new protocol was formulated increasing the amount of Leucocytes and Platelets 10 fold from the standard injectable PRF protocols. The C-PRF protocol sets a new benchmark in what to expect from a Liquid PRF product and forms the foundation of exciting new products like bio-graft, bio-bone, and bio-filler.
Solid PRF membranes for wound healing
Our research team has created a novel method for evaluating and quantifying cell types in platelet-rich fibrin, which has led to key findings on cell content and distribution in a fibrin clot. This new understanding led to the development of the Bio-PRF fibrin clot protocol named Solid PRF. In this, the cell content of the fibrin clot is 4 times higher compared to standard fixed-angle centrifugation systems and protocols. Add to this a clotting sequence that creates a Solid PRF clot after 8 minutes of centrifugation.
Bio-Heat, extending the resorption properties
One of the main limitations of PRF has been it’s short in vivo turnover rate. However, by combining knowledge about how the heating of platelet-poor plasma (PPP) denature and reorganize albumin, with the C-PRF protocol, our research team created the Bio-Heat technology setup that vastly widens the potential of PRF in regenerative medicine by extending resorption properties for up to 4–6 months.
Equine Bio-PRF Protocols
and Future Perspectives
C-PRF – a highly concentrated form of injectable PRF(10x), allowing even larger amounts of regenerative cells to be injected into joints and tendons.
Solid PRF – autologous fibrin membranes encapsulating vast amounts of regenerative cells, slowly releasing pro-angiogenic cytokines to accelerate the healing of even problematic leg ulcers.
BioHeat – The Bio-Heat system is the first commercially available heating system extending the resorption properties of PRF from 2-3 weeks to 4-6 months
Solid PRF – In veterinary ophthalmic surgery PRF seams to serve very well as a scaffold for the healing of corneal injuries combining the reservoir of different growth factors, cytokines and lots of other molecules necessary for proper healing including various nutrients with suturable properties.
Liquid-PRF & Solid PRF – Combination enabling the production of PRF membranes of various sizes. These membranes are flexible and can be sutured offering new perspectives in wound healing and e.g. Gastrogastrointestinal surgery.
BioFiller – The ability to produce a slowly resorbable platelet-rich fibrin filler loaded with regenerative cells may offer an interesting new perspective in Equine Sports Medicine and beyond, as a carrier of medicine or for filling cavities or prolonging the effect of joint injections.
Get started using Equine Bio-PRF
Equine Sports Medicine Starter Package
Blood drawing accessories
Solid PRF box and instruments for membranes
Tubes for C-PRF and Solid PRF
Price 3100 EURBuy online
See Research Papers & Literature
To get an overview of the ongoing research in Bio-PRF please follow this link and see published and articles in progress.
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